Western blot analysis of Glutathione Peroxidase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. GPX1 Polyclonal Antibody (Product # PA5-119990) at a 1:500 dilution was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse liver tissue lysate, untreated . Lane 2: Human liver tissue lysate, untreated.